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aPDT – Reducing the use of Antibiotics in Aquaculture

(Ongoing Project)

Aim/Objective :- To reduce the use of anti-biotic in shrimp larviculture systems. Description :- Extensive use of antibiotics in shrimp farms contributes to the development of antibiotic-resistant pathogens and that these microbes infect both humans and other aquatic organisms. Amid ban in most of the countries and despite negative impact, aquaculturists continue to use antibiotics owing to the lack of viable alternatives with rapid action to combat sudden disease outbreaks/emergencies.

Last year, US Food and Drug Administration has refused 15000 tonnes of shrimp for use of banned antibiotics. Out of that, 3000 tonnes of shrimp were exported from India totalling the loss of USD 264 lakh

Partners :- CSIR-NIO (National Institute of Oceanography), Goa Conclusion :-  Antimicrobial photodynamic therapy (aPDT) Reactor reduces the Vibrio load in Artemia nauplii. The growth of a multiple antibiotic resistant Vibrio harveyi strain was effectively controlled by treating the cells with Rose Bengal and photosensitizing for 30 min using a halogen lamp. This resulted in the death of > 50% of the cells within the first 10 min of exposure and the 50% reduction in the cell wall integrity after 30 min.

Further, mesocosm experiments with V. harveyi and Artemia nauplii demonstrated that in 30 min, the aPDT could kill 78.9% and 91.2% of heterotrophic bacterial and Vibrio population respectively. In conclusion, aPDT with its rapid action and as yet unreported resistance development possibilities could be a propitious strategy to reduce the use of antibiotics in shrimp larviculture systems and thereby, avoid their hazardous effects on human health and the ecosystem at large. Future Scope/ Ongoing Work :- Extending its use cases in all types of aquaculture farms widespread across different part of the world.